Genuin Biotechnologies- One Stop Solutions for Gene Silencing

​At Genuin Biotechnologies LLC, we use our unique ShGE™ gene silencing platform to achieve high throughput, low-cost verification of antibody specificity. Our platform uses lentivirus-mediated short hairpin RNA (shRNA) to silence or knock-down (KD) the messenger RNA (mRNA) encoded by a specific gene, thereby causing the protein encoded by the gene to disappear or become significantly down-regulated. If an antibody can recognize a signal in a wild-type (WT) cell or its lysate, but the signal disappears or significantly decreases in the KD cell or its lysate, the antibody is considered to be highly specific. Based on the above principle, we verify and screen antibodies using techniques such as Western blotting (WB), immunocytochemistry (IC), and flow cytometry (FCM).