Our unique and proprietary lysis buffer formulation (catalog #415) can not only perform all the protein extraction you are currently doing, but further serves as the ultimate solution for extracting large proteins and post-translationally modified (PTM) proteins. ​You do not need to add any inhibitors or phosphatases, or do any sonication. The kit comes in just two easy-to-use parts and works for mammalian cells and tissues (including adipose tissue). Like our other customers, you'll try this and never go back.

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Summary of Key Features:

• Preserve the integrity of large proteins: Near-complete extraction of large proteins; no sonication required means no fragmentation

• Preserve protein PTMs: No loss of protein PTMs even without addition of inhibitors.

• All-in-one formula: No protease/other enzyme inhibitors or sonication required

• Ready-to-use protocol: Simply mix Reagents A & B; extraction takes as little as 15 min

• Wide-range of applications: Suitable for mammalian cells and tissues; equally effective for small and medium proteins