KD-Validated Antibodies
(Partial List)

Antibody specificity refers to the ability of an antibody to bind to and only to its target antigen. This exclusivity requires high-level validation techniques, among which genetic verification is the best choice. Among genetic approaches, gene knockout (KO) at the genomic level and gene knockdown (KD) at the mRNA level using small interference RNA (siRNA) and short hairpin RNA (shRNA) techniques have been widely used for antibody validation. Using KO for a gene leads to the complete ablation of its encoded protein, making it lethal for genes essential to cell survival. Using siRNA to knock down genes is cost-effective and less time-consuming, but the knockdown is transient in and of itself.  Compared to KO and siRNA approach, shRNA KD offers the following benefits, 1) lentiviral shRNA can be stably integrated into a cell’s genome and result in a more complete knockdown compared to siRNA; 2) It is particularly powerful for difficult-to-target and nondividing cells; 3) It knocks down rather than knocks out a gene, so it is particularly useful for the study of genes vital to cell survival.

 

At GenuIN Biotech LLC, we have developed a proprietary shRNA-mediated gene knockdown platform to test antibody specificity. Using our lentiviral platform, we have tested the specificity of antibodies using Western blotting, immunocytochemical staining, or flow cytometry. We validate the specificity of these antibodies so that you don’t need to do it from scratch, saving your resources and time. 

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Species: H, human; M, mouse; R, rat; B, bovine; D, dog, C, chicken; P, pig

Applications: WB, Western blotting; IC, Immunocytochemistry; FCM, Flow cytometry; IHC, Immunohistochemistry