Anti-V5-Tag Mouse Monoclonal Ab#T105

V5-Tag; V5 Epitope Tag

Adding a polypeptide, small protein, or enzyme to a gene of interest, also referred to as protein tagging, is a powerful and versatile technique widely used in biochemistry and molecular biology. In practice, an epitope tag is cloned in-frame relative to the gene of interest, either at the amino (N) or carboxy (C) terminus of the gene. As a result, a fusion protein is synthesized with the peptide tag.

Three-Letter Sequence: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr

One-Letter Sequence: GKPIPNPLLGLDST

Clonality: Mouse monoclonal antibody

Clone ID: 21GB198

Species Reactivity: All

Applications Tested: Western blotting (WB)

Supplied in PBS (pH 7.5) containing 50 µg/ml BSA, 50% glycerol, and 0.01% sodium azide.  

Store at -20 °C for one year.

Western blotting (WB): 1:1,000

• Protein purification
• Detection of the fusion protein, particularly useful when there is no specific antibody to the protein of interest
• Subcellular visualization of the fusion protein
• Western blotting (WB), immunocytochemistry (IC), and (co-)immunoprecipitation

#T105 V5-Tag DS