Pre-Stained Tricolor Protein Ladder#L012
$99.00Price
Background
Protein ladder, also referred to as protein molecular weight marker, is widely used in gel electrophoresis and immunoblotting applications. Our pre-stained tricolor protein ladder provides a convenient way to monitor protein separation and transfer efficiency and allows accurate determination of protein molecular weight on SDS-PAGE gels.
Product Features
- Two high-intensity reference bands (25 and 75 kD)
- Excellent lot-to-lot performance consistency
- Ready-to-use format, no need to reduce, heat, or add loading dyes
- Individually calibrated to ensure consistent band migration and intensity
- Wide range of molecular weights (11-245 kDa)
Number of Bands
12
Band Weights
245, 180, 135, 100, 75, 63, 48, 35, 25, 20, 17, and 11 kDa
Colors
Blue, green (25 kDa), and red (75 kDa)
Visualization
Colorimetric
Recommended Gel
SDS-PAGE
Recommended Membrane
PVDF, nylon, and nitrocellulose
Recommended Volume
3-5 μL for mini gel; 6-10 μL for large gel
Storage
–20°C upon receipt
Applications
- SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
- Western blot (immunoblotting)
Protocol
- Thaw the ladder at room temperature.
- Mix thoroughly by vortexing or inverting the vial 3-5 times.
- Load 3-5 μL per well for mini gel, and 6-10 μL per well for large gel.
Data Sheet